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. Author manuscript; available in PMC: 2012 Apr 5.
Published in final edited form as: Langmuir. 2011 Mar 14;27(7):4257–4264. doi: 10.1021/la105016a

Figure 5.

Figure 5

Illustration of structural differences in gel types. The left side of the figure is representative of mixing step 1 prior to the formation of amide bonds via EDC chemistry and the right side represents the product resulting from mixing step 2 (see also Table 1). The progressive improvement in EPC capture yield and purity from gel types III-VII is attributed to the two step mixing protocol where PEG and antibody are pre-mixed before the introduction of alginic acid and the coupling reagents EDC and sulfo-NHS. Pre-mixing allows optimal dispersion of antibody molecules among the PEG chains.