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Characterization of semisynthetic proteins. Analytical RP-HPLC (top) and ESI-MS (bottom) of modified H2B analogs, 4a–c. The sequence of N-terminal region of each construct is shown beneath the ESI-MS with modified residues highlighted in bold and the underlined alanine representing site of desulfurization. ESI-MS: 4a (M+H)+ observed: 13,900 ± 4 Da; expected: 13,897 Da; 4b (M + H)+ observed: 14,069 ± 5 Da; expected: 14,066 Da; 4c (M + H)+ observed: 13,988 ± 3 Da; expected: 13,986 Da).
