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Ligation strategy used to generate semi-synthetic H2B. (i) EPL between a synthetic peptide α-thioester, corresponding to the N-terminal tail of H2B, and a recombinant protein bearing an N-terminal cysteine and corresponding to the remainder H2B. (ii) Raney-nickel reduction to convert the cysteine residue at the ligation site back to a native alanine.
