Fig. 1.

CBB-stained polyacrylamide gel and intergenic sequence alignment. (A) SDS sample preparation was carried out as described under the Materials and methods. 0.72, 0.76, 0.86 µg protein of samples, respectively, from strains UA159, MT8148, and Z1 were applied to a 5–20% gradient SDS-polyacrylamide gel. The arrow indicates the positions of the 190 kDa bands corresponding to the SpaP proteins. (B) Depicted multiple aligned intergenic (IG) sequences start with the termination codons of the p40f (SMU.609) genes and end with the ATG initiation codons of the spaP genes in UA159IG, MT8148IG (AB548069), and Z1IG (AB516671) sequences. Each sequence is numbered +1 at the first nucleotide of the spaP ATG codon. Lines over the UA159IG and lines under the Z1IG sequences, respectively, indicate −35 and −10 regions of the putative promoter sequences. Boxes indicate direct repeat sequences.