Table 1. List of PCR primers for MYOC (myocilin) exon sequencing in black South African individuals.
| Myocilin exon | Forward primer sequence | Reverse primer sequence | PCR product size (bp) | Covered genomic region* |
|---|---|---|---|---|
| Exon1a |
ATCTTGCTGGCAGCGTGAA |
TCTCTGGTTTGGGTTTCC |
614 |
chr1:171,621,342–171,621,955 |
| Exon1b |
GACAGCTCAGCTCAGGAAGG |
GAAGGTGATCGCTGTGCTTT |
663 |
chr1:171,620,991–171,621,653 |
| Exon2 |
AGCAAAGACAGGGTTTCACC |
AGGGCTTTGTTAGGGAAAGG |
554 |
chr1:171,607,517–171,608,071 |
| Exon3a |
CCCAGACGATTTGTCTCCAG |
TCCCAGGTTTGTTCGAGTTC |
648 |
chr1:171,605,327–171,605,974 |
| Exon3b | GAGAAGGAAATCCCTGGAGC | TGGTGACCATGTTCATCCTTC | 598 | chr1:171,604,914–171,605,511 |
The covered genomic regions were based on the February 2009 human reference sequence (GRCh37).