Table 3.

Parameters characterizing the thermal unfolding of VHP and hydrophobic variants in 50 mM sodium phosphate, pH 7.0 ^a

Variant	Δ Hmb (kcal mol−1)	Δ Smc (cal mol−1 K−1)	Tmd (°C)	Δ Tme (°C)	Δ(ΔG) f (kcal mol −1)
WT	30	88	74.4	-	-
L2A	23	70	60.4	− 14.0	− 1.0
F7A	18	56	41.7	− 32.7	− 2.3
V10A	30	89	67.0	− 7.4	− 0.5
F11A	22	70	48.8	− 25.6	− 1.8
M13A	15	47	34.7	− 39.7	− 2.8
F18A	20	65	28.3	− 46.1	− 3.3
L21A	22	68	45.3	− 29.1	− 2.1
L29A	24	74	55.3	− 19.1	− 1.4
L35A	29	86	67.2	− 7.2	− 0.5

^aThe thermal denaturation curves were analyzed as described. ⁹⁸

^bEnthalpy of unfolding at T_m. The error is ± 2 kcal mol⁻¹.

^cΔ H_m/T_m. The error is ± 5 cal mol⁻¹ K⁻¹.

^dMidpoint of the unfolding curve. The error is ± 0.5 °C.

^eΔ T_m = T_m (variant) − T_m (WT).

^fΔ(ΔG) = ΔT_m × the average Δ S_m value of WT and the variants (71 cal mol⁻¹ K⁻¹).