Table 4.
Variant | ΔHmb (kcal mol−1) | Δ Smc (cal mol−1 K−1) | ΔTmd (°C) | Δ(ΔG) e (kcal mol −1) |
---|---|---|---|---|
WT | 92 | 286 | - | - |
L8A | 89 | 283 | − 5.0 | − 1.34 |
L11A | 78 | 252 | − 11.9 | − 3.08 |
L19A | 73 | 236 | − 13.8 | − 3.65 |
L21A | 85 | 265 | − 1.6 | − 0.41 |
I22V | 74 | 234 | − 5.7 | − 1.48 |
I58V | 89 | 277 | 0.11 | 0.00 |
I70V | 87 | 274 | − 3.4 | − .94 |
I71V | 80 | 249 | − 0.3 | − 0.13 |
L91A | 91 | 288 | − 3.2 | − 0.84 |
I92V | 84 | 266 | − 3.9 | − 1.00 |
The thermal denaturation curves were analyzed as described. 98 The data in the table is the average value of three experiments.
Enthalpy of unfolding at Tm. The error is ± 5 kcal mol−1.
Δ Hm/Tm. The error is ± 15 cal mol−1 K−1.
Δ Tm = Tm (variant) − Tm (WT). The Tm for WT is 48.4 °C.40
Δ(ΔG) = ΔTm × the average Δ Sm value of WT and the variants (264 cal mol−1 K−1).