Table 2. Percent recovery of cytokines diluted in buffer or extracted from spiked Schirmer strips.
|
|
Recovery in assay buffer |
Recovery in extraction buffer |
||
|---|---|---|---|---|
| Cytokine | Diluted sample (Mean/SE) | Extracted SS sample (Mean/SE) | Diluted sample (Mean/SE) | Extracted SS sample (Mean/SE) |
| IL-1α |
77.8±4.7 |
66.9±4.0 |
84.6±5.6 |
79.8±4.2 |
| IL-1β |
81.5±6.2 |
59.0*±3.8 |
84.5±4.3 |
82.9±4.6 |
| IL-1rα |
86.0±4.5 |
67.7±3.5 |
74.5±5.3 |
68.5±3.5 |
| IL-4 |
96.9±8.7 |
37.2±3.5 |
62.9±5.1 |
6.7±1.4 |
| IL-6 |
82.5±3.5 |
68.2±2.7 |
78.2±3.3 |
67.1±3.3 |
| IL-8 |
91.1±5.3 |
68.3*±4.3 |
89.3±4.9 |
76.8±4.2 |
| IL-10 |
83.0±3.2 |
30.5*±2.8 |
BLD |
BLD |
| IL-13 |
83.8±5.0 |
49.7*±2.6 |
85.2±8.2 |
29.4*±3.5 |
| MCP-1 |
90.1±4.4 |
77.7±4.1 |
87.6±4.4 |
84.2±2.5 |
| TNF-α | 81.9±5.8 | 62.8±2.8 | 91.5±7.0 | 65.1*±4.3 |
*p<0.05 versus cytokine diluted in same assay buffer and calculated from same buffer standard curve. BLD: Below the limit of detection. n=10 for all data.