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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Eur J Immunol. 2010 Jul;40(7):1950–1962. doi: 10.1002/eji.200940079

Figure 2.

Figure 2

Evaluation of the ability of three agonist peptides (TV9p6, TV9p5 and TV9p29) listed in Table I to induce antigen-specific, TV9-crossreactive CTLs by in vitro immunization of naïve CD8+ T cells from two to four healthy seronegative donors. Cultures are labeled as TV9pX-Y, where TV9pX identifies the inducing peptide and the numeral Y denotes a distinct donor. (A) TV9p6-6, TV9p6-2, TV9p6-5 and TV9p6–7; (B) TV9p5-3 and TV9p5-4; and, (C) TV9p29-3 and TV9p29-8. CD8+T cells were assayed for specificity and cross-recognition of TV9 using chromium release assays at effector:target (E:T) ratios ranging from 20:1 to 0.6:1. T2 cells without peptide were included as negative controls in each assay. Data are shown as mean ± SEM of triplicate assays and are representative of two independent experiments; in most cases, the error bars are smaller than the plot symbols.