Figure 3.

Specificity and crossreactivity of TV9p6 CTLs monitored over time by tetramer staining. TV9p6-tetramer binding by the CD8⁺ cultures TV9p6-2, TV9p6-6, TV9p6–7 and TV9p6–8 at three time points is shown in Panels A-a to A–c, B-a to B–c, C-a to C-c and D-a to D-c. Crossreactivity of the TV9p6-cultures was assessed concomitantly by staining with TV9-tetramer (A–d to A–f, B–d to B–f, C–d to C–f and D–d to D–f). For comparison, TV9-tetramer staining of the CD8⁺ parallel cultures TV9-2, TV9-6, TV9-7 and TV9-8 is shown in Panels A–g, B–g, C–g and D–g, respectively. Numbers in the upper right quadrant represent the percentage of CD8⁺ tetramer⁺ stained cells. An irrelevant tetramer was used to gate for specific tetramer binding. Data are representative of at least three independent experiments.