Figure 5.
Functional sensitivity for two sets of parallel cultures determined by IFN-γ secretion. (A) TV9-2, (B) TV9p6-2, (C) TV9-8 and (D) TV9p6–8 cultures were stimulated at an E:T ratio of 1:10 with T2 cells pulsed with either TV9 or TV9p6. IFN-γ secretion into the supernatant was quantified by ELISA after 48 hours. (E) EC50 values were calculated for the TV9 and TV9p6 peptides in each case with Graphpad Prism V software. Data are shown as mean ± SEM of triplicate assays and are representative of two independent experiments; in most cases, the error bars are smaller than the plot symbols.