Figure 6.

TV9p6-CTLs suppress HIV replication at least as efficiently as TV9-CTLs. (A) Percent suppression of NL4-3.1 replication in T1 cells at day 3 post-infection by tetramer⁺ T cells at different E:T ratios as indicated. Data are shown as mean ± SEM of triplicate assays and are representative of two independent experiments. (B) Suppression of NL4-3.1 virus in T1 cells by TV9- and TV9p6-CTL cultures. The average percent suppression by TV9-2, -6, -7 and -8 T cells at day 3 and 6 are indicated by ▲ and Δ, respectively. Likewise, suppression mediated by TV9p6-2, -6, -7 and -8 T cells at the two time points are denoted by the symbols ● and ○, respectively. The E:T ratio for this assay was 2:1. Data are representative of two independent experiments. The concentrations of p24 in infected T1 cell cultures in the absence of CTLs were 0.3 ± 0.03 ng/mL and 0.8 ± 0.1 ng/mL on day 3 and 93 ± 14 ng/mL and 684 ± 48 ng/mL on day 6. (C) Suppression of HIV JR-CSF replication in two acutely infected, allogeneic HLA-A*0201-matched CD8-depleted PBMC cultures by two parallel CTL cultures (TV9-6 and TV9p6-6; TV9-7 and TV9p6–7) at day 9 post-infection. The concentrations of p24 in infected PBMC-1 and PBMC-2 cultures in the absence of CTLs were 311 ng/mL and 210 ng/mL, respectively. The p values comparing suppression by TV9- and TV9p6-CTL were determined using an unpaired, two-tailed Student T test with Graphpad Prism V software.