Table I.
Recognition of predicted candidate peptides, presented by T2 target cells, by the index and four allogeneic HLA-A*0201+ TV9-CTL cultures as determined by cytotoxicity. Positive recognition for TV9 and reactive artificial sequences is boldfaced and shaded. Nd, not determined.
| Candidate Peptide Name and Sequence |
Residues Substituted |
TV9-1 (index) | TV9-2 | TV9-3 | TV9-4 | TV9-5 | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| % Lysis at Peptide Concentrations (ng/mL) |
|||||||||||||||||||||||||||||||
| 1 | 10 | 100 | 1000 | 1 | 10 | 100 | 1000 | 1 | 10 | 100 | 1000 | 1 | 10 | 100 | 1000 | 1 | 10 | 100 | 1000 | ||||||||||||
| Native | TV9 | T | L | N | A | W | V | K | V | V | 0 | 3 | 18 | 42 | 50 | 19 | 73 | 83 | 81 | 7 | 34 | 64 | 76 | 50 | 78 | 85 | 78 | 7 | 35 | 59 | 44 |
| Group I | p30 | – | I | – | – | – | I | – | – | – | 2 | 0 | 6 | 29 | 45 | 2 | 36 | 80 | 75 | 2 | 37 | 56 | 60 | 0 | 5 | 58 | 53 | 0 | 36 | 67 | 76 |
| p6 | K | I | – | – | – | I | – | – | – | 3 | 0 | 8 | 31 | 41 | 34 | 73 | 77 | 78 | 10 | 34 | 51 | 51 | 0 | 0 | 30 | 45 | 2 | 8 | 52 | 76 | |
| p29 | – | I | – | – | – | I | – | G | – | 3 | 15 | 37 | 47 | 64 | 29 | 63 | 77 | 74 | 9 | 40 | 48 | 59 | 6 | 57 | 68 | 55 | 5 | 48 | 56 | 86 | |
| p5 | K | I | – | – | – | I | – | G | – | 4 | 0 | 20 | 44 | 51 | 64 | 84 | 84 | 85 | 14 | 30 | 30 | 34 | 1 | 0 | 25 | 53 | 8 | 4 | 13 | 58 | |
| p42 | – | Q | – | – | – | I | – | – | – | 2 | 2 | 7 | 27 | 41 | 9 | 63 | 75 | 74 | 0 | 24 | 46 | 48 | 0 | 9 | 59 | 83 | 2 | 2 | 29 | 28 | |
| p18 | K | Q | – | – | – | I | – | – | – | 3 | 0 | 11 | 29 | 44 | 29 | 66 | 75 | 73 | 7 | 28 | 41 | 46 | 0 | 0 | 43 | 81 | 0 | 5 | 35 | 49 | |
| p41 | – | Q | – | – | – | I | – | G | – | 3 | 13 | 32 | 42 | 52 | 47 | 79 | 84 | 77 | 8 | 31 | 40 | 53 | 0 | 37 | 65 | 80 | 0 | 3 | 28 | 28 | |
| p17 | K | Q | – | – | – | I | – | G | – | 4 | 4 | 33 | 44 | 61 | 66 | 83 | 86 | 82 | 11 | 24 | 29 | 28 | 0 | 15 | 69 | 87 | 0 | 2 | 3 | 14 | |
| p40 | – | Q | – | – | – | I | – | E | – | 3 | 0 | 17 | 39 | 46 | 33 | 73 | 78 | 70 | 17 | 26 | 32 | 34 | 0 | 0 | 0 | 1 | 0 | 5 | 5 | 14 | |
| p16 | K | Q | – | – | – | I | – | E | – | 4 | 0 | 21 | 36 | 50 | 6 | 52 | 76 | 80 | 23 | 23 | 28 | 30 | 0 | 0 | 0 | 0 | 2 | 1 | 6 | 13 | |
| p35 | – | I | – | W | – | I | – | G | – | 4 | 1 | 3 | 24 | 47 | 0 | 1 | 6 | 35 | 0 | 0 | 0 | 0 | 79 | 79 | 81 | 77 | 0 | 2 | 36 | 41 | |
| p47 | – | Q | – | W | – | I | – | G | – | 4 | 4 | 17 | 30 | 47 | 2 | 6 | 30 | 54 | 0 | 0 | 0 | 7 | 56 | 83 | 85 | 83 | 0 | 2 | 5 | 18 | |
| p23 | K | Q | – | W | – | I | – | G | – | 5 | 0 | 2 | 24 | 43 | 1 | 0 | 4 | 11 | 0 | 0 | 0 | 0 | 76 | 92 | 90 | 93 | 0 | 2 | 5 | 18 | |
| Group II | p28 | – | I | – | – | – | I | – | E | – | 3 | 0 | 1 | 14 | 46 | 19 | 64 | 77 | 72 | 31 | 37 | 34 | 37 | 0 | 0 | 0 | 0 | 0 | 14 | 25 | nd |
| p36 | – | I | – | W | – | I | – | – | – | 3 | 3 | 0 | 0 | 4 | 3 | 8 | 15 | 43 | 0 | 0 | 0 | nd | 34 | 66 | 78 | 73 | 0 | 4 | 8 | 29 | |
| p34 | – | I | – | W | – | I | – | E | – | 4 | 0 | 0 | 0 | 0 | 2 | 3 | 3 | 12 | 0 | 0 | 0 | 4 | 1 | 13 | 56 | 67 | 0 | 0 | nd | 7 | |
| p4 | K | I | – | – | – | I | – | E | – | 4 | 0 | 0 | 19 | 47 | 43 | 72 | 79 | 74 | 25 | 32 | 32 | 34 | 0 | 0 | 0 | 1 | 8 | 0 | 9 | 36 | |
| p12 | K | I | – | W | – | I | – | – | – | 4 | 0 | 0 | 0 | 3 | 1 | 9 | 15 | 51 | 0 | 0 | 2 | 11 | 33 | 83 | 87 | 89 | 0 | 3 | 16 | 31 | |
| p10 | K | I | – | W | – | I | – | E | – | 5 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 11 | 37 | 1 | 0 | 10 | nd | |
| p11 | K | I | – | W | – | I | – | G | – | 5 | 0 | 0 | 0 | 30 | 0 | 4 | 13 | 16 | 0 | 0 | 0 | 0 | 57 | 84 | 84 | 82 | 3 | 2 | 14 | nd | |
| p39 | – | Q | – | – | – | F | – | – | – | 2 | 0 | 0 | 0 | 11 | 2 | 2 | 30 | 52 | 0 | 0 | 1 | 20 | 0 | 0 | 1 | 19 | 0 | 7 | 23 | 52 | |
| p37 | – | Q | – | – | – | F | – | E | – | 3 | 0 | 0 | 0 | 16 | 0 | 0 | 16 | 37 | 0 | 16 | 26 | 32 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 7 | |
| p38 | – | Q | – | – | – | F | – | G | – | 3 | 0 | 6 | 26 | 38 | 0 | 0 | 12 | 32 | 0 | 0 | 1 | 17 | 0 | 3 | 38 | 68 | 0 | 6 | 9 | 47 | |
| p15 | K | Q | – | – | – | F | – | – | – | 3 | 0 | 0 | 17 | 40 | 15 | 66 | 69 | 69 | 0 | 0 | 7 | 35 | 0 | 0 | 5 | 27 | 1 | 8 | 15 | 38 | |
| p13 | K | Q | – | – | – | F | – | E | – | 4 | 0 | 0 | 0 | 29 | 0 | 12 | 48 | 57 | 1 | 19 | 28 | 29 | 0 | 0 | 0 | 0 | 6 | 6 | 9 | 14 | |
| p14 | K | Q | – | – | – | F | – | G | – | 4 | 0 | 0 | 8 | 36 | 19 | 8 | 38 | 72 | 0 | 0 | 0 | 17 | 0 | 0 | 9 | 42 | 5 | 0 | 4 | 14 | |
| p45 | – | Q | – | W | – | F | – | – | – | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 54 | 0 | 14 | 22 | 27 | 31 | 77 | 84 | 79 | 0 | 0 | 0 | 1 | |
| p43 | – | Q | – | W | – | F | – | E | – | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 7 | 12 | 65 | 74 | 82 | 0 | 4 | 0 | 2 | |
| p44 | – | Q | – | W | – | F | – | G | – | 4 | 0 | 0 | 0 | 32 | 0 | 1 | 0 | 4 | 0 | 0 | 0 | 3 | 46 | 71 | 76 | 83 | 0 | 0 | 0 | 4 | |
| p21 | K | Q | – | W | – | F | – | – | – | 4 | 0 | 0 | 0 | 0 | 3 | 0 | 10 | 48 | 0 | 20 | 29 | 39 | 43 | 89 | 89 | 95 | 1 | 4 | 9 | 16 | |
| p19 | K | Q | – | W | – | F | – | E | – | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 47 | 87 | 83 | 0 | 3 | 11 | 23 | |
| p20 | K | Q | – | W | – | F | – | G | – | 5 | 0 | 0 | 0 | 22 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 54 | 96 | 85 | 91 | 0 | 6 | 9 | 19 | |
| p48 | – | Q | – | W | – | I | – | – | – | 3 | 0 | 0 | 0 | 3 | 2 | 7 | 20 | 51 | 0 | 0 | 0 | 10 | 32 | 68 | 84 | 83 | 0 | 3 | 20 | 5 | |
| p46 | – | Q | – | W | – | I | – | E | – | 4 | 0 | 0 | 0 | 1 | 3 | 2 | 16 | 31 | nd | 2 | 27 | 34 | 40 | 78 | 86 | 87 | 0 | 0 | 5 | 6 | |
| p24 | K | Q | – | W | – | I | – | – | – | 4 | 0 | 0 | 0 | 3 | 0 | 1 | 15 | 31 | 0 | 0 | 4 | 17 | 34 | 66 | 92 | 90 | 0 | 2 | 4 | 9 | |
| p22 | K | Q | – | W | – | I | – | E | – | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 3 | 0 | 0 | 12 | 52 | 2 | 6 | 6 | 29 | |
| p27 | – | I | – | – | – | F | – | – | – | 2 | 0 | 0 | 0 | 14 | 1 | 4 | 33 | 61 | 0 | 0 | 3 | 21 | 0 | 0 | 0 | 5 | 1 | 5 | 37 | nd | |
| p25 | – | I | – | – | – | F | – | E | – | 3 | 0 | 1 | 0 | 7 | 1 | 1 | 7 | 14 | 0 | 0 | 0 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | nd | |
| p26 | – | I | – | – | – | F | – | G | – | 3 | 0 | 0 | 0 | 23 | 0 | 6 | 12 | 27 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 36 | 0 | 1 | 7 | nd | |
| p3 | K | I | – | – | – | F | – | – | – | 3 | 0 | 0 | 0 | 34 | 12 | 63 | 68 | 58 | 0 | 0 | 0 | 15 | 0 | 0 | 0 | 0 | 1 | 0 | 31 | 68 | |
| p1 | K | I | – | – | – | F | – | E | – | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 37 | 0 | 0 | 0 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 10 | |
| p2 | K | I | – | – | – | F | – | G | – | 4 | 0 | 0 | 0 | 13 | 0 | 6 | 52 | 64 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 10 | 19 | |
| p33 | – | I | – | W | – | F | – | – | – | 3 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 13 | 0 | 4 | 29 | 36 | 9 | 47 | 73 | 77 | 0 | 0 | 13 | 6 | |
| p31 | – | I | – | W | – | F | – | E | – | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 64 | 64 | 0 | 7 | 7 | 13 | |
| p32 | – | I | – | W | – | F | – | G | – | 4 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 8 | 0 | 0 | 0 | 1 | 19 | 59 | 84 | 78 | 0 | 4 | 5 | 20 | |
| p9 | K | I | – | W | – | F | – | – | – | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 6 | 29 | 38 | 0 | 23 | 79 | 86 | 8 | 9 | 13 | nd | |
| p7 | K | I | – | W | – | F | – | E | – | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 14 | 7 | 4 | 3 | 40 | |
| p8 | K | I | – | W | – | F | – | G | – | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 0 | 0 | 0 | 0 | 18 | 81 | 87 | 0 | 5 | 9 | 43 | |
| No. of the 48 artificial sequences recognized | 14 | 16 | 6 | 28 | 6 | ||||||||||||||||||||||||||