Strategy of tissue-specific RNAi using the rde-1 mutant
A: Yellow colour shows RNAi resistant cells. Red colour shows RNAi sensitive cells. Expression of rde-1 in tissue A should result in RNAi sensitivity in tissue A, but not tissue B.
B: Plasmids used in this study. The lin-26 promoter (3.7 kb 5′ sequence) was placed upstream of NLS-GFP or rde-1, resulting in hypodermis-specific NLS-GFP or rde-1 expression. The myo-3 promoter (2.4 kb 5′ sequence) was fused to NLS-GFP, resulting in a muscle-specific NLS-GFP expressing plasmid (pPD93.97). The hlh-1 promoter (3.0 kb 5′ sequence) was fused to rde-1, resulting in a muscle-specific rde-1 expressing plasmid.