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. Author manuscript; available in PMC: 2011 May 3.
Published in final edited form as: J Periodontal Res. 2008 Apr;43(2):201–211. doi: 10.1111/j.1600-0765.2007.01013.x

Figure 5. RAW 264.7 differentiated into TRAP+, multinucleated osteoclastic-like cells in response to exogenous RANKL or co-cultured with LPS-stimulated PDL cells.

Figure 5

Top panel depicts RAW 264.7 cells only (5 × 104) cultured for 6 days without or with RANKL (50ng/mL) and RANKL/OPG (100ng/mL). Middle panel indicate co-culture experiments where vehicle control or LPS (1 μg/mL) treated mPDL cells were stimulated for 72h, then 5 × 104 cells were added to each well containing 5 × 104 RAW 264.7 cells. Co-cultures were maintained for 6 days, both in the presence and absence of OPG (100ng/mL) (right panels). All images are representative of three independent experiments.