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. 2011 Feb 10;32(5):695–702. doi: 10.1093/carcin/bgr027

Fig. 5.

Fig. 5.

IL-1β is responsible for the induction of COX-2. (A) HCC1954 and THP-1 cells were cocultured for the indicated time periods. Levels of IL-1β in CM were determined utilizing enzyme-linked immunosorbent assay. Data are mean ± standard deviation (n = 3). (B) HCC1954 cells were cocultured with THP-1 cells or treated with 1 h coculture CM in the absence or presence of IL-1Ra for 6 h, and levels of COX-2 and β-actin in HCC1954 cell lysate were determined by western blot. (C) HCC1954 cells were cocultured with THP-1 cells or treated with 1 h coculture CM in the absence or presence of IL-1β-neutralizing antibody (IL-1β Ab) or mouse IgG1 for 6 h, and levels of COX-2 and β-actin in HCC1954 cell lysates were determined. (D) HCC1954 cells were cocultured with THP-1 cells for 1 h in the absence or presence of IL-1Ra. ROS levels were measured in HCC1954 cells. Data are mean ± standard deviation (n = 3). (E) RT-4 cells were cocultured with THP-1 cells or incubated with 1 h coculture CM in the presence or absence of IL-1Ra, and levels of COX-2 and β-actin in RT-4 cell lysates were determined.