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. Author manuscript; available in PMC: 2012 Feb 25.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Jan 31;405(4):662–666. doi: 10.1016/j.bbrc.2011.01.089

Figure 4. E2-25K Polyubiquitin Chain Assay.

Figure 4

SDS-PAGE of Polyubiquitin Chain Assays. Lane 1 – LMW Marker, Lane 2 – E2-25K, Lane 3 – E2-25K UBC Domain, Lane 4 – Assay with E2-25K and wt Ub, Lane 5 – Assay with E2-25K and equimolar ratios of Ub-K48C and Ub-D77, Lane 6 – Assay with E2-25K and Ub-K48C only, Lane 7 – Assay with E2-25K UBC Domain and wt Ub, Lane 8 – Assay with E2-25K UBC Domain and equimolar ratios of Ub-K48C and Ub-D77, Lane 9 – Assay with E2-25K UBC Domain and Ub-K48C only.