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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: J Eukaryot Microbiol. 2010 Aug 5;57(5):405–414. doi: 10.1111/j.1550-7408.2010.00496.x

Fig. 2.

Fig. 2

Confocal microscopic examination of Leishmania major rosettes labeled with Poly α 2,8 N-acetyl neuraminic acid (PSA) and PSA containing de-N-acetyl neuraminic acid (NeuPSA). A, light micrograph with DAPI-fluorescent labeling overlay; B, anti-PSA mAb 2-1-B/DAPI labeling; C, anti-NeuPSA mAb SEAM 2/DAPI labeling; D, composite of mAb 2-1-B/mAb SEAM 2/DAPI labeling. Individual promastigote cells within the rosette expressing PSA, NeuPSA or both can be observed. Scale bars, 10 µm.