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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: J Eukaryot Microbiol. 2010 Aug 5;57(5):405–414. doi: 10.1111/j.1550-7408.2010.00496.x

Fig. 4.

Fig. 4

Specific inhibition of anti-Poly α 2,8 N-acetyl neuraminic acid (anti-PSA) and anti-PSA containing de-N-acetyl neuraminic acid (anti-NeuPSA) mAb binding to leishmanial rosettes. Monoclonal antibodies (mAbs) were preincubated with each inhibitor for 20 min and subsequently added to rosette preparations. Poly α 2,8 N-acetyl neuraminic acid (PSA) is reactive with mAbs 2-1-B and SEAM 12 but not mAb SEAM 2; N-propionyl PSA (N-Pr PSA) is reactive with mAbs SEAM 12 and SEAM 2 but not mAb 2-1-B; N-trichloroacetyl PSA (N-TcAc PSA) is reactive only with mAb SEAM 2. Labeled cells were examined by flow cytometry. Black fill, indicates mAb binding; gray fill, indicates mAb signal in the presence of specific inhibitor; white fill, irrelevant isotype matched control mAb. Specific inhibition of each anti-PSA and anti-NeuPSA mAb is observed.