Fig. 5.
Temperature dependency of cell surface expression of Poly α 2,8 N-acetyl neuraminic acid (PSA) and PSA containing de-N-acetyl neuraminic acid (NeuPSA) by leishmanial rosettes. Cells were cultivated at 16 °C (dotted line, white fill), 20 °C (black fill), and 24 °C (dotted line, gray fill) for at least 4 weeks prior to labeling. Irrelevant isotype matched control mAb (solid line, white fill, signal intensity ≤10). Maximal cell surface expression of PSA/NeuPSA occurs when promastigotes were cultured at 20 °C.