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. Author manuscript; available in PMC: 2011 May 3.
Published in final edited form as: Science. 2011 Mar 11;331(6022):1289–1295. doi: 10.1126/science.1198830

Figure 4.

Figure 4

(A and B) Images of two FOVs taken at three different wavelengths with triple label extract to monitor U1-DHFR/Cy5-TMP and U2-SNAP-DY549 association with Alexa488-labeled pre-mRNA, without (A) or with (B) ATP. (C) Magnification of dashed area in (B) showing co-localization of U1 (yellow boxes) with U2 (white spots). (D) Fluorescence intensity traces showing association of U1 and U2 with an individual pre-mRNA molecule (not shown) in the presence of ATP. Arrival times for each subcomplex (tU1 and tU2) are marked. (E) Histogram of the delay between subcomplex arrival times (tU2-tU1). (F, H) Single molecule fluorescence intensity traces for U2/U5 (F) and U5/NTC (H) bound to single pre-mRNA molecules (not shown). (G, I) Histograms of the delays between U2 and U5 binding (G) and U5 and NTC binding (I).