Effects of silencing MUC1 on proliferation of KMS28PE cells. A, Lysates from KMS28PE cells stably infected with lentiviruses expressing the control siRNA (A and B clones), MUC1siRNA#1 or MUC1siRNA#2 were immunoblotted with anti-MUC1-C and anti-ß-actin. B, Nuclear lysates from the indicated KMS28PE cells were immunoblotted with anti-ß-catenin and, as controls for loading and purity, with antibodies against nuclear lamin B and cytosolic IκBα. Whole cell lysate (WCL) was used as a control for IκBα. C and D, Proliferation of the KMS28PE cell clones CsiRNA-A (solid circles), CsiRNA-B (solid squares), MUC1siRNA#1 (open circles) and MUC1siRNA#2 (open squares) in 10% (C) and 2% (D) FBS.