Table I.
Gene | Forward primers | Reverse primers | Fragments | TaqMan probe or SYBR Green |
---|---|---|---|---|
MUC1 | 5'-GCCAGGATCTGTGGTGGTACA-3' | 5'-CTCCAGGTCGTGGACATTGA-3' | 70-bp | 6FAM-TTGACTCTGGCCTTCCCGAGAAGGTACC-3'TAMRA |
TRIM46 | 5'-CACTGGACAAATCATTCAACAACTT-3' | 5'-AAACATTATCTCACACCATCCTCTCA-3' | 127-bp | 6FAM-TGCCCTGCAATGTCCATACCTAAAGGGT-3'TAMRA |
THBS3 | 5'-GCTTAAATAGTGACCACCGGGT-3' | 5'-AACGCTGCTCATGGAAGCC-3' | 65-bp | SYBR Green |
GAPDH | 5'-GGTTTACATGTTCCAATATGATTCCA-3' | 5'-ATGGGATTTCCATTGATGACAAG-3' | 93-bp | 6FAM-ATGGCACCGTCAAGGCTGAGAACG-3'TAMRA |