Figure 7.

The inhibitory effect of surfactant protein D (SP-D) on eosinophil chemotaxis can be modulated by S-nitrosylation. Eosinophils isolated from peripheral blood of allergic patients were assayed for migration toward eotaxin (positive controls), medium alone (negative control), oligomeric (native) rrSP-D, or S-nitrosylated rrSP-D combined with medium or eotaxin. The data are expressed as a percent of migrated eosinophils toward testing solutions, where 100% was calculated as the number of cells migrating toward medium only. ^#P < 0.05, basal migration toward eotaxin control compared with the same treatment; *P < 0.05, migration toward eotaxin control compared with rrSP-D or S-nitrosothiol–SP-D treatment. All test solutions were used in triplicate, n = 5 individual experiments.