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. Author manuscript; available in PMC: 2011 May 3.
Published in final edited form as: Anal Biochem. 2004 Mar 1;326(1):1–12. doi: 10.1016/j.ab.2003.11.007

Fig. 3.

Fig. 3

Identification of muropeptide classes by size-exclusion chromatography. Peptidoglycan was digested with N-acetylmuramidase SG and labeled with ANTS. The resulting muropeptides were applied to a Biogel P-6 column, fractionated, and the muropeptides were detected as described under Materials and methods. (A) Muropeptides from digested peptidoglycan of E. coli CS703-1 were eluted from the column and detected by absorbance at 208 nm. Arrows indicate fractions selected for FACE analysis shown in B. (B) Muropeptide composition of six fractions from A, detected by FACE. The fraction number is given below each lane. T, trimer muropeptides; D, dimer muropeptides; M, monomer muropeptides; dye, background of residual ANTS fluorophore common to each sample.