Table 3.
Muropeptide class | Fluorophore (%) |
||
---|---|---|---|
ANSA | ANDA | ANTS | |
Trimers | 9.3±1.1 | 111.7±0.7 | 9.8±0.6 |
Dimers | 34.7±2.9 | 33.1±4.1 | 35.2±1.9 |
Monomers | 44.8±3.6 | 45.4±4.0 | 45.6±1.8 |
E. coli CS109 peptidoglycan was labeled as described in the legend to Fig. 2, and the relative percentages of muropeptide bands of each size class in FACE gels were quantified by using the one-dimensional band analysis module of Quantity One software. Data represent the mean±standard deviation of two separate experiments.