Table 7.
Muropeptide composition of E. coli PBP mutants
| Muropeptides | CS109 | CS617-1K | CS703-1 |
|---|---|---|---|
| Wild type | Δ1 a 4 6 7 CH | Δ1 a 4 5 6 7 CH | |
| Monomers | 43.2±2.6 | 26.8±1.9 | 23.0±2.1 |
| Dimers | 34.9±2.9 | 47.2±1.9 | 48.8±2.9 |
| Trimers | 10.5±1.4 | 15.2±1.3 | 15.1±0.7 |
| Monomers | |||
| Tripeptide | 11.8±0.7 | 5.5±0.4 | 7.1±0.5 |
| Tetrapeptide | 30.7±1.4 | 20.7±0.9 | 11.1±0.4 |
| Pentapeptide | 0.7±0.2 | 0.6±0.1 | 4.8±0.3 |
The percentages of muropeptides in these E. coli strains represent the mean±standard deviation from measurements of three different FACE gels. Amounts of muropeptides that are significantly different from those of E. coli CS109 are underlined: solid underline (higher), dotted underline (lower). The PBPs deleted from each strain are listed under the strain name: numbers represent specific PBP designations; C, AmpC; H, AmpH.