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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Ann Neurol. 2011 Jan;69(1):47–64. doi: 10.1002/ana.22308

FIGURE 4.

FIGURE 4

Overexpression of SorCS1 decreased the secretion of total APPs and sAPPα into culture media. CM and total cell lysate from HEK293 APPsw transfected with SorCS1 isoforms were analyzed by western blot with antibodies against APP (22C11 and 8717), Aβ (6E10), and SorCS1 (R&D). Total sAPP and sAPPα from CM were decreased compared with XL4 in spite of similar FL-APP expression in cell lysate. Western blot was done on the same samples with β -tubulin antibody (loading control). The expression of both SorCS1 isoforms in lysate was similar. APP = amyloid precursor protein; APPsw = amyloid precursor protein Swedish mutation; CM = conditioned media; FL-APP = APP holoprotein; sAPPα = α-secretase cleavage; XL4 = empty vector.