Figure 4. Effect of time in culture on tissue biochemical parameters. A: MTT incorporation; an indicator of tissue viability, B: LDH release; an indicator of tissue integrity, C: total tissue glutathione content as a function of time in culture. Media was replaced at 24 h. Data represent mean ± SEM from triplicate determinations in placental tissue from 3 patients (patients 3, 4, 5 for LDH release and total glutathione measurements; patients 6, 7, 8 for MTT incorporation). * represents p<0.05 vs. each of the other time points and † represents p<0.05 vs. the 4 h time point when compared by repeated measures one-way ANOVA followed by Tukey’s post test for all possible pair wise comparisons; all other comparisons were not significant.