Table 1.
Primers for deletions at the vicinity of the GGC1274–1276 codon
| Plasmid (deleted region) |
Primer name |
Primer sequence |
|---|---|---|
| pNTF-D1 | D1F | 5'-AAAGGTACCTCCAGGGCACCCCGAAT-3' |
| (Δ1243–1268) | ||
| pNTF-D2 | D2F | 5'-ATCCAGGGCACCGGAGAGCTTCCCGGAG-3' |
| (Δ1280–1288) | D2R | 5'-GGGAAGCTCTCCGGTGCCCTGGAT-3' |
| pNTF-D3 | D3F | 5'-ACCCCGAATTCGCGGCTCTGCCCTCTTG-3' |
| (Δ1289–1339) | D3R | 5'-AGGGCAGAGCCGCGAATTCGGGGTGCCCTG-3' |
| pNTF-D4 | D4F | 5'-GTGACCGCTTCCATGGTGGGTGAAGAG-3' |
| (Δ1340–1396) | D4R | 5'-TTCACCCACCATGGAAGCGGTCACAGAG-3' |
| pNTF-D5 | D5F | 5'-AAAGGTACCATGGTGGGTGAAGAGAAG-3' |
| (Δ1243–1396) | ||
| pNTF-D6 | D6F | 5'-ACCCCGAATTCGGACCTTCCTGCTG-3' |
| (Δ1289–1306) | D6R | 5'-AGCAGGAAGGTCCGAATTCGGGGTG-3' |
| pNTF-D7 | D7F | 5'-CTTCCCGGAGTCTCTGTGACCGCTTC-3' |
| (Δ1307–1324) | D7R | 5'-AGCGGTCACAGAGACTCCGGGAAG-3' |
| pNTF-D8 | D8F | 5'-CCTGCTGGCTGCCGGCTCTGCCCTCTT-3' |
| (Δ1325–1339) | D8R | 5'-AGGGCAGAGCCGGCAGCCAGCAGGAAG-3' |