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. Author manuscript; available in PMC: 2011 May 3.

Published in final edited form as: Biochem J. 2010 Mar 29;427(2):265–274. doi: 10.1042/BJ20091589

HEK293 and SMS-KCNR cells were labeled with C12-dhCCPS for 1 hour and then treated with the indicated concentration of hydrogen peroxide for 1h (A,B) or with 200μM hydrogen peroxide for the indicated time (C,D). Percent of conversion of C12-dhCCPS into C12-CCPS (A,C) as well as levels of endogenous dihydroceramide (B,D) were measured by LC/MS. * p<0.05, **p<0.01, ***p<0.001.

HEK293 and SMS-KCNR cells were labeled with C12-dhCCPS for 1 hour and then treated with the indicated concentration of hydrogen peroxide for 1h (A,B) or with 200μM hydrogen peroxide for the indicated time (C,D). Percent of conversion of C12-dhCCPS into C12-CCPS (A,C) as well as levels of endogenous dihydroceramide (B,D) were measured by LC/MS. * p<0.05, **p<0.01, ***p<0.001.