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. Author manuscript; available in PMC: 2011 May 3.
Published in final edited form as: Biochem J. 2010 Mar 29;427(2):265–274. doi: 10.1042/BJ20091589

Table 1.

Effect of hydrogen peroxide on ceramide and dihydroceramide species in HEK293 cells treated with 200μM H2O2 for 1h.

dhC14-
Cer		 dhC16-
Cer		 dhC18-
Cer		 dhC20-
Cer		 dhC22-
Cer		 dhC22:1-
Cer		 dhC24-
Cer		 dhC24:1-
Cer		 dhC26-
Cer		 dhC26:1-
Cer		 DHSph Sph
Control 0.00072±0.00007 0.01106±0.002 0.00103±0.0003 0.00344±0.0007 0.00077±0.00009 0.00182±0.00023 0.00160±0.0002 0.00131±0.00014 0.00037±0.00014 0.00005±0.00002 0.000812±0.00015 0.00479±0.0004
H2O2 0.00290*±0.00154 0.05528**±0.01802 0.00216**±0.00052 0.00440±0.00047 0.00386**±0.00129 0.00197±0.00024 0.01288***±0.00165 0.00974**±0.00352 0.00043±0.00042 0.00030**±0.00011 0.00095±0.00025 0.00387 ±00038
C14-Cer C16-Cer C18-Cer C18:1-Cer C20-Cer C20:1-Cer C22-Cer C22:1-Cer C24-Cer C24:1-Cer C26-Cer C26:1-Cer
Control 0.00168±0.00026 0.14530±0.02186 0.01659±0.00267 0.00382±0.00063 0.02039±0.00229 0.00234±0.00036 0.03420±0.00546 0.00301±0.00007 0.10791±0.01305 0.07417±0.00624 0.00187±0.00016 0.00169±0.00062
H2O2 0.00207±0.00038 0.14767±0.03153 0.01984±0.00087 0.00480±0.00056 0.02172±0.00281 0.00301±0.00066 0.03843±0.00480 0.00329±0.00029 0.10328±0.00891 0.06998±0.00517 0.00216*±0.00011 0.00169 ±0.00025

Values are shown as pmoles/nmole of Pi and are mean ±S.D. of three independent experiments done in duplicate.

*

p<0.05,

**

p<0.01,

***

p<0.001.