Figure 3. Volcano plot used in differential expression analysis.

The x-axis is log₂ ratio of gene expression levels between two stages; the y-axis is adjusted p value based on −log₁₀. The colored dots (L1 = green) and right (L3i = red) represent the differentially expressed genes based on p<0.01 (False Discovery Rate = 2.5%; represented by black horizontal line) and 2-fold expression difference (represented by two black vertical lines).