Table 1. Experimentally validated signalogs and their orthologs.

Selected signalogs (gene name)	ORFs (WormBase ID)	Mutant allele	Signalog class	Known signaling ortholog in fly	Known signaling ortholog in human	Role of the ortholog in the Notch pathway
aqp-6	C32C4.2	lf mutant: (tm2407)V	3	bip	-	co-factor
crb-1	F11C7.4	lf mutant: (ok931)X	3	crb	NOTCH2	receptor
D1009.3	D1009.3	lf mutant: (ok1349)X	1	sca	-	co-factor
F10D7.5	F10D7.5	no characterized mutant alleles	4	neur	NEURL	transcription factor, co-factor
nsh-1	F20H11.2	no characterized mutant alleles	2	sno	SBNO1	transcription factor
num-1	T03D8.1	lf mutant: (ok433)V	4	numb	NUMB	co-factor

We experimentally validated 6 novel Notch pathway components in C. elegans. 4 of them have been characterized by at least one loss-of-function mutant allele. None of these mutations caused altered morphology, behavior, or survival, i.e., these mutant animals appear superficially wild-type. Fly and human orthologs are also listed. Signalogs are classified into 5 groups (signalog classes) based on their known properties (see Methods and Fig. 3a). In the SignaLink database, each protein is annotated with one or more pathway role (e.g., co-factor, receptor).