Table 2. Summary of ITC experiment using resorcinol titrating RolR and mutant proteins.

	Kd (µM)	Relative affinity	n	ΔH (cal·mol−1)	ΔS (cal·mol−1·K−1)
RolR	0.19±0.03	100%	1.02±0.005	−1.990×104±189.8	−36.0
D94A	3.7±0.51	5.1%	0.990±0.016	−1.648×104±356.3	−30.4
R145A	6.7±0.71	2.8%	0.969±0.014	−1.509×104±295.9	−26.9
R148A	2.2±0.29	8.6%	1.05±0.011	−1.926×104±321.0	−38.6
D149A	-	0	-	-	-

The parameter for D149A binding resorcinol is too weak to be determined (Kd >1 mM).