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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Int Immunopharmacol. 2011 Jan 18;11(7):859–869. doi: 10.1016/j.intimp.2011.01.004

Fig. 2.

Fig. 2

Digital radiography of tumors in the hind legs of nude mice. Mice were injected intraperitoneally with PBS, PBS liposomes, or clodronate liposomes one day prior to the intratibial injection of 2 × 105 PC-3MM2, PC-3MM2-NS shRNA, or PC-3MM2-IL-6 shRNA cells. Extensive bone lysis was found in mice with PC-3MM2 and PC-3MM2-NS shRNA tumors treated with PBS or PBS liposomes. There was significant preservation of bone in mice injected with cells transfected with IL-6 shRNA (PC-3MM2-IL-6 shRNA) and mice treated with clodronate liposomes. The most important outcome (no lysis) was achieved by the combination of knockdown of IL-6 in tumor cells and treatment of mice with clodronate liposomes.