Skip to main content
. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Int Immunopharmacol. 2011 Jan 18;11(7):859–869. doi: 10.1016/j.intimp.2011.01.004

Fig. 3.

Fig. 3

Western blot and immunohistochemical analyses. A: Cell lysates were harvested from LNCaP, PC3-MM2, PC-3MM2-NS shRNA, and PC-3MM2-IL-6 shRNA cells and analyzed by Western blot for the expression of p44/p42 and phosphorylated p44/p42, and tumor tissues were stained with anti-phosphorylated antibody to determine the expression of phosphorylated p44/p42. There was no significant difference in the expression of p44/p42 or phosphorylated p44/p42 among cultured cells PC-3MM2, PC-3MM2-NS shRNA, and PC-3MM2-IL-6 shRNA. The expression of p44/p42 or phosphorylated p44/p42 did not differ between androgen-dependent/PSA producing cells (LNCaP) and androgen-independent/PSA non-producing cells (PC-3MM2) growing in culture. B: The expression of phosphorylated p44/p42 was significantly reduced in PC-3MM2-IL-6 shRNA tumors growing in vivo.