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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Melanoma Res. 2011 Jun;21(3):180–187. doi: 10.1097/CMR.0b013e3283456dfc

Figure 3.

Figure 3

Resveratrol deactivates and attenuates Akt. Exponentially growing B16F10 cells were treated with (A) DMSO or 100µM resveratrol (RES) and with (B) DMSO, 100µM resveratrol (RES) or 25 µM Ly294002 (LY) in serum-free media for the designated time. Equal amounts of protein were loaded onto polyacrylamide gels, transferred to PVDF membrane and immuno-stained with an anti-phospho (S473) AKT antibody, an anti-phospho (T308) AKT antibody or an anti-Pan Akt antibody that recognizes the three isoforms of Akt. GAPDH was used as a loading control. The figure is representative of three separate experiments.