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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Melanoma Res. 2011 Jun;21(3):180–187. doi: 10.1097/CMR.0b013e3283456dfc

Figure 4.

Figure 4

Inhibition of Akt degradation partially rescues cell migration. A) Exponentially growing B16F10 cells were incubated with or without 100 µM resveratrol in the presence or absence of 50 µM Boc- D -Fmk for the designated time in serum-free media. Lysates were prepared and equal amounts of protein were subjected to SDS-PAGE, blotted and immuno-stained with anti-Pan Akt antibodies. GAPDH was stained as a loading control. Data from three separate experiments were quantified using Image-J software and presented relative to resveratrol at time zero. Mean values ± S.D. B) In a transwell assay, photomicrographs of 4 different fields were compiled. The number of cells were counted for the three conditions - DMSO, 100 µM resveratrol, and 100 µM resveratrol supplemented with 50 µM Boc-D –Fmk. The representative outcome from three separate experiments is shown. Mean values ± S.D.