Figure 6.

Resveratrol treatment reduces tumor burden and metastasis in syngeneic mouse models of melanoma. A) B16BL6 cells were inoculated subcutaneously at day zero. Treatment with 50 mg/kg resveratrol in Neobee oil or vehicle alone by daily oral gavage began on day 4. Tumor measurements were described in Methods. Panel B illustrates the Western analysis of Akt levels in tumor specimens obtained from the mice described in (A) treated with either resveratrol or vehicle. In a separate experiment, mice were inoculated with cells through the tail vein and treated i.p. either with resveratrol or vehicle alone as described in Methods. Histological sections from the lungs of resveratrol-treated (C) and untreated (D) mice were stained with H&E, and tumors were demarcated using ImageJ software and their areas calculated.