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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Clin Cancer Res. 2011 Mar 17;17(9):2874–2884. doi: 10.1158/1078-0432.CCR-10-2334

Figure 3. All breast cancer cell lines express high levels of eIF4E both in the nucleus and the cytoplasm.

Figure 3

(A) eIF4E protein levels in whole cell extracts from exponentially growing cells were assessed by western blot, which showed elevated levels in all tumor cell lines. (B) Immunofluorescence staining of eIF4E (green) in BT474, MDA-MB-468, MDA-MB-231 and ZR75.1 cells shows that eIF4E is present in the nucleus as well as the cytoplasm.