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Guanidine hydrochloride denaturation of headpiece constructs. GuHCl-induced denaturation of HP67 (■), MetMet (▲), SWAP (◯), and K70M (▽). Experiments were performed with 10 μM protein samples at 25 °C in 10 mM sodium phosphate buffer, pH 7.0. CD signal was monitored at 222 nm in a 10 mm cell. Data was best fit to the Santoro and Bolen equation (34) and then plotted as percent unfolded.
