Skip to main content
. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Mol Microbiol. 2011 Feb 10;80(1):14–32. doi: 10.1111/j.1365-2958.2011.07558.x

Figure 3.

Figure 3

CaMtw1 is needed for high fidelity chromosome segregation in C. albicans. (A) CAKS12 (PCK1pr-MTW1/mtw1) cell viability drops dramatically between 4 h and 6 h after repression of CaMTW1 expression on glucose. CAKS12 cells were grown overnight on succinate media, washed and shifted to glucose media. Cells were then harvested at 2 h intervals. Cell viability was calculated by plating diluted aliquots of culture at indicated time points on succinate plates and counting the number of colonies that appeared after incubating the plates for 3 days at 30°C. (B) Images of CAKS11 (MTW1/mtw1) and CAKS12 cells harvested at the indicated time points. (C) Distribution of unbudded (G1), small budded (S) and large budded (G2/M) cells of CAKS11 and CAKS12 after media shift from succinate to glucose. (D) FACS profiles of CAKS11 and CAKS12 cells incubated in glucose media for indicated time points and stained with propidium iodide. The X axis uses propidium iodide staining intensity as a measure of DNA content and the Y axis reports the relative number of cells.