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. 2011 May 2;193(3):445–454. doi: 10.1083/jcb.201101134

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© 2011 Rossio and Yoshida

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Localization of Zds1 and Cdc55. (A) Cell cycle localization of endogenous Zds1-GFP. Cells were fixed with formaldehyde and stained with DAPI. (B) Cell cycle localization of endogenous Cdc55-GFP. In live cells, DNA was stained with Hoechst 33258. (C) Nuclear localization of Cdc55-GFP is affected in zds1Δ zds2Δ cells and in the cells overexpressing Zds1 (GAL1-ZDS1). (D) Nuclear accumulation of Cdc55-GFP is prevented by Zds1 and Zds2. The ratio of mean fluorescence intensity of nuclear (Nuc) and cytoplasmic (Cyt) Cdc55-GFP in each individual cell was plotted. Horizontal lines indicate means. n = 8–13 for each category. G1, unbudded cells; G2, budded with a single nucleus; M, large budded cells with dividing or divided nuclei. P-value was calculated by Student’s unpaired t test.