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. 2011 May 2;193(3):445–454. doi: 10.1083/jcb.201101134

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© 2011 Rossio and Yoshida

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Figure 3. — Zds1/Zds2 and cytoplasmic Cdc55 promote mitotic entry. (A) Serial dilutions of indicated strains spotted on YPD (yeast peptone dextrose) at different temperatures. (B) Representative images of cells with the indicated genotypes in the presence (top row) or absence (bottom row) of SWE1 except for cdc55-NES zds1Δ zds2Δ. Cells were grown in YPD media at 24°C. (C) Our model of mitotic entry regulation by Zds1/Zds2 and cytoplasmic Cdc55. (D) Cytoplasmic Cdc55 is required for dephosphorylation of Cdc28-Y19. Cells were treated with 15 µg/ml nocodazole for 3 h to prevent mitotic progression before cell lysate extraction for Western blotting. Antiphospho–Cdc2-Y15 and antitubulin (loading control) antibodies were used for detection. P, phosphorylation. WT, wild type.