Table 4.
Effects of silica gel column chromatography fractions of methanol extracts from microalgae against the release of EBV in Akata, B95-8, and P3HR-1 cell lines based on the amount of cell-free EBV DNA detected by real-time PCR
| Cell line | Silica gel fraction | IC50 (μg/ml) | EC50 (μg/ml) | Therapeutic index |
| Akata | Ankistrodesmus convolutus | |||
| ACF1 | >200 | >20 | ND | |
| ACF2 | 35±1 | 4.2±1.1 | 8.3 | |
| ACF3 | >200 | 6.0±1.2 | >33 | |
| ACF4 | 77±8 | >20 | <4 | |
| ACF5 | 87±14 | >20 | ND | |
| ACF6 | 51±2 | 19.7±0.8 | 3.0 | |
| ACF7 | >200 | >20 | ND | |
| Synechococcus elongatus | ||||
| SEF1 | >200 | >20 | ND | |
| SEF2 | >200 | 16.9±0.4 | >12 | |
| SEF3 | 22±2 | 9.6±0.4 | 2.0 | |
| SEF4 | >200 | >20 | ND | |
| SEF5 | >200 | >20 | ND | |
| SEF6 | >200 | >20 | ND | |
| SEF7 | >200 | >20 | ND | |
| B95-8 | Ankistrodesmus convolutus | |||
| ACF1 | 68±24 | 3.1±0.8 | 22.0 | |
| ACF2 | >200 | >20 | ND | |
| ACF3 | 94±12 | >20 | <5 | |
| ACF4 | >200 | >20 | ND | |
| ACF5 | >200 | 6.2±3.4 | >32 | |
| ACF6 | >200 | 4.4±2.2 | >46 | |
| ACF7 | >200 | >20 | ND | |
| Synechococcus elongatus | ||||
| SEF1 | 89±13 | 3.5±2.5 | 32.0 | |
| SEF2 | 91±6 | >20 | <5 | |
| SEF3 | 40±1 | 3.2±0.4 | 12.5 | |
| SEF4 | >200 | 15.7±4.7 | >13 | |
| SEF5 | >200 | >20 | ND | |
| SEF6 | >200 | >20 | ND | |
| SEF7 | >200 | >20 | ND | |
| P3HR-1 | Ankistrodesmus convolutus | |||
| ACF1 | >200 | 13.5±3.0 | >15 | |
| ACF2 | 55±1 | >20 | <3 | |
| ACF3 | >200 | >20 | ND | |
| ACF4 | >200 | >20 | ND | |
| ACF5 | >200 | 18.7±1.0 | >11 | |
| ACF6 | 70±2 | >20 | <4 | |
| ACF7 | >200 | >20 | ND | |
| Synechococcus elongatus | ||||
| SEF1 | >200 | 2.9±0.9 | >69 | |
| SEF2 | 76±4 | 13.1±4.3 | 6.0 | |
| SEF3 | 24±6 | 3.8±1.3 | 6.0 | |
| SEF4 | >200 | >20 | ND | |
| SEF5 | >200 | >20 | ND | |
| SEF6 | >200 | >20 | ND | |
| SEF7 | >200 | >20 | ND | |