Figure 7.

Dexrazoxane (DRZ) induces the expression of antiapoptotic genes. Immunoblot analysis of cytosolic extracts of untreated H9c2 cells (C), and cells exposed for 24 h to doxorubicin (DOX), for 3 h to dexrazoxane alone or pretreated with DRZ for 3 h and then exposed to DOX. When appropriate, the cells were also transfected with the expression vector ΔARNT. In some cases, the cells were exposed to DMOG for 24 h or to BSO and H₂O₂ for 3 h, and then washed and re-incubated for 2 h. Antibodies against the indicated proteins were used, and the blots were reprobed using the antibody against α-tubulin as a loading control. The panels show one representative blot and the densitometric quantification relative to C-values. Mean values ± SD. *P < 0.001; **P < 0.005, n = 3. DMOG, dimethyloxalyl glycine; BSO, buthionine sulphoximine.