Figure 1.

Functional responses obtained with different combinations of wild type (α6, β2 and β3) and modified (α6/3, β3^V273S) nAChR subunits following transient transfection into HEK293T cells, as tested in the FLIPR Ca²⁺ influx assay. Upon addition of 500 µM acetylcholine (A) or 200 nM epibatidine (B) at 10 s, normalized responses (as % of basal fluorescence units) were recorded. Subunit combination labels are above the corresponding curves. Traces from the subunit combinations that were inactive or poorly active, including α6β2β3, α6β2β3^V273S, β2β3 and control plasmid transfection, overlie and are un-labelled. CoTxMII, cells were co-incubated with 10 µM α-conotoxin MII.