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Effects of high-fat diet and metformin treatment on relaxant responses to acetylcholine (A) and sodium nitroprusside (B) in aortic segments from GK rats, compared with those from nondiabetic Wistar rats, after phenylephrine preconstriction. Relaxation of aortic rings was measured using an isometric force displacement transducer. Data are expressed as mean ± SE (n = 6–16). ***P < 0.001 versus Wistar group; §§§P < 0.001 versus GK control group; φφφP < 0.001 versus GK + AD group.
