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. Author manuscript; available in PMC: 2011 May 4.
Published in final edited form as: Environ Sci Technol. 2008 Jul 15;42(14):5254–5259. doi: 10.1021/es703186e

FIGURE 1.

FIGURE 1

PrPTSE extraction efficiency from aerobic and anaerobic sludge using 1% SDS. Brain homogenate (BH) or proteinase K (PK)-treated BH was incubated with each sludge and extracted using 1% SDS and vortexing. Extracted protein was compared to starting material by immunoblot analysis and densitometry. A minimum of three independent replicates were used for each experiment. (A) Extraction from aerobic sludge, (B) Extraction from anaerobic sludge, (C) Densitometric measurements of extraction of full-length and truncated PrP from each sludge relative to the highest densitometric value observed. Error bars represent the standard deviation of these densitometric measurements. A.U. = absorbance units